A4471

Immunoblotting
Extract of 293T cells transfected with ARTS plasmid was separated on SDS-PAGE and probed with Monoclonal PA to ARTS (MO) CL:ART S51 (Cat. No. A4471). The antibody was developed with Alkaline Phosphatase conjugate (Cat. No. A2179) and a NBT/BCIP substrate.
Antibody dilution 1/4,000.

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A4471

Sigma

 

Monoclonal Anti-ARTS antibody produced in mouse

~2 mg/mL, clone ARTS51, purified immunoglobulin, buffered aqueous solution

Synonym:Anti-Apoptosis-Related Protein in the TGF-β Signaling Pathway
MDL number:MFCD03096625

References

Reviews

Description

Immunogensynthetic peptide corresponding to C-terminal region (amino acids 247-266) of human ARTS protein, conjugated to KLH.
Physical formSolution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody formpurified immunoglobulin
cloneARTS51 (monoclonal)
formbuffered aqueous solution
mol wtantigen mol wt 32 kDa
species reactivityhuman
concentration~2 mg/mL
application(s)immunoblotting: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing ARTS
 immunocytochemistry: suitable
 indirect ELISA: suitable
 microarray: suitable
isotypeIgG2b
Gene Informationhuman ... SEPT4(5414)
shipped indry ice
storage temp.−20°C

Safety

WGK GermanyNWG

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